![]() |
||||||||||||||
Australia: The Land Where Time Began |
||||||||||||||
Denisova Cave Used by Denisovans for many thousands of Years
DNA sequencing of genetic material extracted from a finger of a young
girl that had been found in Denisova Cave in the Altai Mountains of
Siberia resulted in the discovery of a new type of hominin, Denisovans.
A small number of Denisovan molars were found in the cave and together
with new dates for the material show that the cave was being occupied by
the Denisovans over a very long period. It is suggested by the data that
the girl lived about 50,000 BP and 2 other Denisovan individuals died in
the cave at least 110,000 years ago and possibly as long ago as 170,000
BP. The new dates have helped to improve the murky view of Denisovans,
who left very little evidence of their presence, and provide more
convincing evidence of their multiple occupations of the cave over a
very long period, according to Fred Spooner, a paleoanthropologist.
Layer 11, a thick sandstone band, is where most of the key fossils in
the cave have been found. The first attempt to date the animal bones and
artefacts from the sandstone layer varied widely between 30,000 and
50,000 BP. When 20 samples of artefacts and animal bones that had cut
marks indicating they had been butchered by the occupants of the cave
gave ages on a second dating of likely older than 48,000-50,000 BP. The
sediments at the base of the sandstone layer, where the finger bone was
discovered, gave dates at the very limit of radiocarbon dating, so are
probably older.
The dates that had been obtained were from bones with cut marks, and
ornaments, and the dates were consistent across 3 caves, as well as
fitting with genetic evidence.
Nuclear DNA sequencing was carried out on 3 molars recovered from layer
11 and the molar of a child recovered from a deeper layer, 22.
Thermoluminescence, a dating method that is considered to be
experimental in the case of cave dating, gave a date of 170,000 BP for
layer 22.
A significant amount of nuclear DNA from 3 teeth that was analysed
proved to be from Denisovans, and a 4th was Neanderthal. When
key sites on the tooth DNA were compared with corresponding sites in the
high quality genomes from the Denisovan girl, Neanderthal and modern
humans, it was revealed that the Denisovan inhabitants of the cave were
not closely related. The Denisovans displayed more genetic variation
among them than the entire DNA that has been sequenced from all the
Neanderthals tested to date, though Neanderthals are known to be
genetically similar.
The entire mtDNA genomes of the Denisovans were sequenced to determine
when the Denisovans were in the cave and they were placed on a family
tree. The number of mtDNA differences between individuals was counted
and the mutation rate of modern humans was used to estimate the length
of time it would require for these mutations to appear. By this method
it was concluded that the girl was in the cave about 65,000 after the
oldest Denisovan, who was there at least 110,000 years ago, though
possibly earlier.
Pääbo’s team has sequenced the DNA from a Neanderthal toe bone and a
molar recovered from the cave. Also, modern humans were apparently in
the cave, which was large and light-filled, as indicated by more recent
artefacts. It appears from the interdigitisation of the Denisovans and
the Neanderthals both groups were in and out of the cave.
A new technique, ZooMS, was used to scan 2,315 bones from the cave
searching for proteins that were uniquely of human origin, which found a
fragment of a human toe bone.
Ancient DNA reveals tryst between extinct human species
2
The woman had a Neanderthal mother and a Denisovan father. She may have
been a teenager when she died more than 50,000 BP. There was enough
ancient DNA in the 2 cm fragment of her finger bone to reveal her
unexpected ancestry: She was the direct offspring of 2 different species
of ancient humans. The report of the genome of the woman that was
published in Nature indicates that her mother was Neanderthal and her
father was Denisovan. It is the most direct evidence so far found that
various ancient humans mated with each other and had offspring.
Researchers had already concluded, based on other ancient genomes, which
Denisovans, Neanderthals, and modern humans interbred in Europe and Asia
in the ice age. Many people of the present carry genes from both archaic
human species. It has been shown by other fossils that were fund in the
Siberian cave that all 3 species lived there at different times.
According to Johannes Krause, an ancient DNA researcher at the Max Plank
Institute, this find is sensational, because they now have the genome of
the love child between 2 different hominin groups, and found in a place
where members of both groups have been found. According to Viviane Slon,
the palaeontologist who carried out the analysis, on seeing the results
her first reaction was disbelief. She repeated the analysis several
times before she and her group were convinced. That the direct offspring
between 2 ancient humans was present among the first few fossil genomes
that were recovered from the cave suggests, according to Svante Pääbo,
that when these groups met, they actually mixed quite freely with each
other.
The characteristics of the fragments of bone suggest it came from
someone who was at least 13 years old. Slon et
al. found that the person the
bone came from was female, and that her genome matched that of
Neanderthal and Denisovan in roughly equal amounts. Also, the proportion
of genes in which her chromosome pairs contained different variants,
heterozygous alleles, was close to 50% for all chromosomes, which
suggests the maternal and paternal chromosomes came from different
groups. And her mitochondrial DNA, which is inherited from the mother,
was uniformly Neanderthal, so it was concluded by the researchers that
she was a first generation hybrid of a Denisovan man and a Neanderthal
woman.
Further study of the genome found that her father also had some
Neanderthal ancestry, possibly from several hundred generations back.
And the woman’s Neanderthal genes are closer to those of a Neanderthal
found in Croatia than those from the Siberian cave. It is suggested by
this that distinct groups of Neanderthals migrated back and forth
between western Europe and Siberia a number of times.
It appears they freely spread their genes to outsiders along the way.
According to Krause that highlights the question of why the Denisovans
and Neanderthals nevertheless remained groups that were genetically
distinct. He posed the question that if they came together from time to
time why didn’t they come together as a single population. The
researchers suggested that a role was probably played by geographic
barriers, though to understand the true legacy of these prehistoric
couplings more fossils with ancient DNA are required.
1.
Gibbons, A. (2015).
"Cave was lasting home to Denisovans." Science 349(6254):
1270-1271.
2.
Vogel, G., 2018,
Science, Ancient DNA reveals tryst between extinct human species
VOL 361 ISSUE 6404
|
|
|||||||||||||
|
||||||||||||||
Author: M.H.Monroe Email: admin@austhrutime.com Sources & Further reading |